Phenylpyruvate reductase and method for manufacturing optically-active phenyllactic acid and 4-hydroxyl-phenyllactic acid using same enzyme
AssigneeAsahi Kasei Chemicals Corporation
InventorsKazunobu Konishi, Naoki Takaya
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Abstract
The present invention provides a phenylpyruvate reductase for efficiently obtaining highly pure, optically active 3-phenyllactate and 4-hydroxyphenyllactate; a gene coding for the same; and a method for manufacturing optically active 3-phenyllactate and 4-hydroxyphenyllactate employing the same.
Claims8
A method of producing D phenyllactate or D-4-hydroxyphenyllactate, comprising
- transforming a microoganism with a recombinant vector containing
- a complementary polynucleotide coding for an amino acid sequence having at least 90% identity to an amino acid sequence shown by SEQ ID NO:
- 4,
- culturing the transformed microorganism with a substrate, and
- recovering D-phenyllactate or D-4-hydroxyphenyllactate from a microorganism culture.
A microorganism designated as Wickerhamia fluorescens TK1, and deposited with the Patent Organism Depository (IPOD) of National Institute of Advanced Industrial Science and Technology under Accession No. FERM BP-11466.
Dependencies are inferred from the claim text.