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AssigneesUniversity College Cork, National University of Ireland, University of Kent
InventorsMichael Prentice, Martin Warren, Mingzhi Liang
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A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is transformed to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolized to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size.
A method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, said method comprising:
A method of removing inorganic phosphate (Pi) from a system,
A recombinant bacteria which is genetically transformed to express a heterologous bacterial microcompartment operon and an enzyme fused to a bacterial microcompartment localization signal, wherein the enzyme is capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, wherein the microcompartment has pores that are dimensioned to allow the low molecular weight substrate pass into the microcompartment and prevent the polymeric or high molecular weight molecular products from passing out of the microcompartment.
A plasmid comprising a nucleic acid sequence encoding an enzyme fused to a bacterial microcompartment localization signal, said enzyme being capable of converting a low molecular weight substrate into a polymeric or high molecular weight molecular weight product and an empty bacterial microcompartment, wherein the plasmid is capable of expressing within a bacterial host an empty bacterial microcompartment and a fusion protein comprising the enzyme and the microcompartment localisation signal.
A kit of parts comprising:
Dependencies are inferred from the claim text.