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US 9,186,843 B2Granted
Cell counting and sample chamber and methods of fabrication
AssigneeNexcelom Bioscience LLC
InventorsLeo L. Chan, Jean Qiu
Life of this patent
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Abstract
The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological sample. More particularly, the invention relates to a unique cell counting chamber, e.g., a thin gap fluidic cell chamber for both bright field and fluorescent imaging of bacteria or parasites, and methods for making the same.
Claims14
A method for fabricating a sample chamber, the method comprising:
- providing an optically clear backing layer;
- placing uniformly an irradiation- or thermally-curable layer on the backing layer;
- imprinting a preformed structure mold on to the irradiation- or thermally-curable layer, wherein the preformed structured mold protruding into the irradiation- or thermal-curable layer;
- curing the irradiation- or thermally-curable layer by irradiation or thermal treatment;
- removing the preformed structure mold, thereby forming an imprinted structure in the irradiation- or thermal-cured layer reflecting the preformed structure of the mold;
- and
- placing an optically clear top film having an optically clear adhesive layer on to the imprinted structure of the irradiation- or thermally-cured layer, thereby forming a chamber having the imprinted structure.
4 more elements
A method for manufacturing a sample chamber suitable for holding a biological sample, the method comprising:
- imprinting a preformed structure mold on to an irradiation- or thermal-curable layer, the preformed structured mold protruding into the irradiation- or thermal-curable layer;
- curing the irradiation- or thermal-curable layer to form an optically clear layer thereby forming a chamber having the imprinted structure;
- removing the preformed structure mold;
- binding an optically clear top film to the imprinted structure of the irradiation- or thermal-cured layer, thereby forming a sample chamber;
- and
- forming one or more access port on the chamber allowing introduction and removal of a sample.
3 more elements
Dependencies are inferred from the claim text.