Methods to modulate lysine variant distribution
AssigneeAbbVie Inc.
InventorsKartik Subramanian, Mayda Perez, Xiaobei Zeng, Chee Furng Wong, Christopher Chumsae
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Abstract
The instant invention relates to the field of protein production and purification, and in particular to compositions and processes for controlling the distribution or amount of lysine variants expressed by host cells, as well as to compositions and processes for controlling the amount of lysine variants present in purified preparations.
Claims30
A method for producing a composition comprising adalimumab, comprising culturing a cell producing adalimumab in a cell culture media comprising an amount of one or more basic amino acids sufficient to produce a composition comprising adalimumab in which less than 62% of the lysine variant species of adalimumab have zero C-terminal lysines (Lys 0).
A method for producing a composition comprising adalimumab, comprising culturing a cell producing adalimumab in a cell culture media comprising an amount of lysine sufficient to produce a composition comprising adalimumab in which less than 62% of the lysine variant species of adalimumab have zero C-terminal lysines (Lys 0).
A method for producing a composition comprising adalimumab, comprising culturing a cell producing adalimumab in a cell culture media comprising an amount of one or more basic amino acids sufficient to produce a composition comprising adalimumab wherein the sum of lysine variant species of adalimumab having one C-terminal lysine (Lys 1) and lysine variant species of adalimumab having two C-terminal lysines (Lys 2) is greater than 35%.
Dependencies are inferred from the claim text.