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US 9,181,566 B2Granted
Genetic switches for butanol production
AssigneeButamax Advanced Biofuels LLC
InventorsMichael Dauner, Arthur Leo Kruckeberg, Robert A Larossa, Brian James Paul, Wonchul Suh, Joseph Frederich Tuminello
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Abstract
The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same.
Claims6
A method of producing butanol or 2-butanone comprising:
- A) contacting a recombinant host cell comprising a heterologous polynucleotide, said heterologous polynucleotide comprising
- i) a promoter nucleic acid sequence, wherein the promoter nucleic acid sequence is selected from SEQ ID NO:
- 775, 776, 777, 778, 772, 773, 768, 769, or 711;
- and
- ii) a nucleic acid sequence encoding a biocatalyst polypeptide, wherein the biocatalyst polypeptide catalyzes a substrate to product conversion of pyruvate to acetolactate;
- with a carbon substrate under a first set of conditions;
- and
- B) contacting the recombinant host cell with a carbon substrate under a second set of conditions;
- wherein the first set of conditions and second set of conditions differ and wherein the nucleic acid sequence encoding a biocatalyst polypeptide is differentially expressed under the first set of conditions than under the second set of conditions;
- and
- wherein the host cell produces butanol or 2-butanone under at least one of the first set or the second set of conditions.
8 more elements
Dependencies are inferred from the claim text.