Microbial fermentation methods and compositions
AssigneeNewLeaf Symbiotics, Inc.
InventorGregg Bogosian
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Abstract
The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
Claims17
A fermentation product comprising a solid substance wherein a mono-culture or co-culture of Methylobacterium is adhered thereto by having been grown on said solid substance, wherein said solid substance comprises a plurality of particles of about 1 micron to about 1000 microns in average length or average diameter and is not a photosynthetic microorganism, and wherein said fermentation product is essentially free of contaminating microorganisms other than the Methylobacterium, and wherein the Methylobacterium titer of said solid substance is at least 5×108 colony-forming units per gram of solid to at least about 5×1013 colony-forming units of Methylobacterium per gram of solid.
A biphasic fermentation broth comprising a liquid phase and a solid substance wherein a mono-culture or co-culture of Methylobacterium is adhered thereto by having been grown on said solid substance, wherein said solid substance comprises a plurality of particles of about 1 micron to about 1000 microns in average length or average diameter and is not a photosynthetic microorganism, wherein said fermentation broth is essentially free of contaminating microorganisms other than the Methylobacterium, and wherein the biphasic fermentation broth comprises Methylobacterium at a titer of 5×108 colony-forming units per milliliter to about 6×1010 colony-forming units per milliliter.
Dependencies are inferred from the claim text.