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US 9,181,540 B2Granted
Method for production of polypeptide
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
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Abstract
The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.
Claims12
A method for producing a desired antibody, comprising
- culturing a cell capable of high-yield production of β-alanine to produce the desired antibody;
- wherein the cell has been transformed by DNA encoding the desired antibody,
- wherein the cell has been transformed by DNA encoding cysteine sulfinic acid decarboxylase or a mutant thereof,
- wherein the mutant has the same enzyme activity as cysteine sulfinic acid decarboxylase, and
- wherein the DNA encoding the cysteine sulfinic acid decarboxylase is selected from the group consisting of:
- (a) a DNA encoding a polypeptide consisting of the amino acid sequence as shown in SEQ ID NO:
- 8;
- (b) a DNA encoding a polypeptide consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
- 8 by substitution, deletion and/or addition of 1-10 amino acid residues, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (c) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO:
- 2, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (d) a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7;
- and
- (e) a DNA that hybridizes to a DNA complementary to a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7 under high stringent conditions of 65° C., 2×SSC and 0.1% SDS, wherein the DNA encodes a polypeptide having cysteine sulfinic acid decarboxylase activity.
13 more elements
A method for producing a desired antibody, comprising
- culturing a cell that strongly expresses cysteine sulfinic acid decarboxylase to produce the desired antibody;
- wherein the cell has been transformed by DNA encoding the desired antibody,
- wherein the cell has been transformed by DNA encoding cysteine sulfinic acid, decarboxylase, and
- wherein the DNA encoding the cysteine sulfinic acid decarboxylase is selected from the group consisting of:
- (a) a DNA encoding a polypeptide consisting of the amino acid sequence as shown in SEQ ID NO:
- 8;
- (b) a DNA encoding a polypeptide consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
- 8 by substitution, deletion and/or addition of 1-10 amino acid residues, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (c) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO:
- 2, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (d) a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7;
- and
- (e) a DNA that hybridizes to a DNA complementary to a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7 under high stringent conditions of 65° C., 2×SSC and 0.1% SDS, wherein the DNA encodes a polypeptide having cysteine sulfinic acid decarboxylase activity.
12 more elements
A recombinant vector comprising a DNA encoding cysteine sulfinic acid decarboxylase selected from the group consisting of:
- (a) a DNA encoding a polypeptide consisting of the amino acid sequence as shown in SEQ ID NO:
- 8;
- (b) a DNA encoding a polypeptide consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
- 8 by substitution, deletion and/or addition of 1-10 amino acid residues, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (c) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO:
- 2, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity;
- (d) a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7;
- and
- (e) a DNA that hybridizes to a DNA complementary to a DNA consisting of the nucleotide sequence as shown in SEQ ID NO:
- 7 under high stringent conditions of 65° C., 2×SSC and 0.1% SDS, wherein the DNA encodes a polypeptide having cysteine sulfinic acid decarboxylase activity.
8 more elements
Dependencies are inferred from the claim text.