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US 9,181,531 B2Granted
Process for purifying VLPs
AssigneeApse, Inc.
InventorsJuan Pedro Humberto Arhancet, Juan P. Arhancet, Kimberly Delaney, Kathleen B. Hall, Neena Summers
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Abstract
Processes and compositions to produce, package, and purify virus like particles containing heterologous cargo molecules utilizing self assembling proteins and protease treatment coupled with simple precipitation and filtration methods are described.
Claims19
A process for purifying a quantity of virus-like particles (VLPs) consisting of Enterobacteriophage MS2 capsid proteins (SEQ ID NO.:
- 3) enclosing at least one heterologous cargo molecule, the process comprising:
- (a) obtaining a cell lysate comprising a plurality of the VLPs;
- (b) contacting the cell lysate with a protease for a time and under conditions sufficient to hydrolyze cell lysis products other than the VLPs to form a hydrolysate;
- and (c) isolating the VLPs from the hydrolysate.
1 more element
Dependencies are inferred from the claim text.