Pharmaceutical compositions for binding sphingosine-1-phosphate
AssigneeLpath, Inc.
InventorsRoger A. Sabbadini, Scott R. Pancoast, Genevieve Hansen, William A. Garland, Marina Safonov
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions that comprise an anti-S1P monoclonal antibody, antibody fragment, or derivative in a carrier, particularly a buffered, hypertonic aqueous solution, as well as to kits containing such compositions, and methods for using such compositions for treatment of diseases and conditions associated with S1P.
Claims12
A composition comprising a pharmaceutically acceptable carrier and an antibody or an antigen-binding antibody fragment that binds sphingosine-1-phosphate (S1P) under physiological conditions, wherein the composition is hypertonic and the antibody or an antigen-binding antibody fragment comprises at least one heavy chain variable domain and at least one light chain variable domain, wherein:
- A. each heavy chain variable domain comprises:
- (i) a first sequence of amino acid residues of sequence DHTIH (SEQ ID NO:
- 4);
- (ii) a second sequence of amino acid residues selected from the group consisting of AISPRHDITKYNEMFRG (SEQ ID NO:
- 6);
- and
- (iii) a third sequence of amino acid residues of sequence GGFYGSTIWFDF (SEQ ID NO:
- 5);
- and
- B. each light chain variable domain comprises the following:
- (i) a first sequence of amino acid residues of sequence ITTTDIDDDMN (SEQ ID NO:
- 1);
- (ii) a second sequence of amino acid residues of sequence EGNILRP (SEQ ID NO:
- 2);
- and
- (iii) a third sequence of amino acid residues of sequence LQSDNLPFT (SEQ ID NO:
- 3),
- wherein the composition comprises a buffered hypertonic solution having a pH between about pH 5.5 and about pH 6.5 and comprising sodium chloride in a concentration of at least about 250mM.
An aqueous composition, comprising:
- a. a pharmaceutically acceptable carrier that comprises a buffered hypertonic solution comprising from about 250 mM to about 3M sodium chloride and having a pH that is between about pH 5.5 and about pH 6.5;
- and
- b. an antibody or an antigen-binding antibody fragment that binds sphingosine-1-phosphate (S1P ) under physiological conditions, wherein the antibody or an antigen-binding antibody fragment is present at a concentration of less than about 100 mg/mL and comprises at least one heavy chain variable domain and at least one light chain variable domain, wherein:
- A. each heavy chain variable domain comprises:
- (i) a first sequence of amino acid residues of sequence DHTIH (SEQ ID NO:
- 4);
- (ii) a second sequence of amino acid residues selected from the group consisting of AISPRHDITKYNEMFRG (SEQ ID NO:
- 6);
- and
- (iii) a third sequence of amino acid residues of sequence GGFYGSTIWFDF (SEQ ID NO:
- 5);
- and
- B. each light chain variable domain comprises the following:
- (i) a first sequence of amino acid residues of sequence ITTTDIDDDMN (SEQ ID NO:
- 1);
- (ii) a second sequence of amino acid residues of sequence EGNILRP (SEQ ID NO:
- 2);
- and
- (iii) a third sequence of amino acid residues of sequence LQSDNLPFT (SEQ ID NO:
- 3).
Dependencies are inferred from the claim text.