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US 9,180,207 B2Granted
Epo knockout GFP anemic mouse
AssigneeTohoku Techno Arch Co., Ltd.
InventorsMasayuki Yamamoto, Naoko Minegishi, Shun Yamazaki
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Abstract
The present invention relates to a model animal spontaneously developing anemia. More specifically, the invention relates to a transgenic non-human mammal spontaneously developing anemia associated with a postnatal decrease in production of erythropoietin (Epo), Epo-producing cells prepared from the transgenic non-human mammal, and a screening method using the Epo-producing cells.
Claims3
A transgenic mouse developing anemia after birth by genetic modification, wherein the genetic modification comprises:
- (a) a homozygous knockout of an endogenous erythropoietin (Epo) gene in the genome of the mouse;
- and
- (b) introduction of an extraneous Epo gene, in a chromosome of the mouse, as a transgene, the transgene being a region from 3.3-kb upstream to 4.5-kb downstream of a transcription start site of the Epo gene,
- wherein:
- the mouse is hemizygous or homozygous for the extraneous Epo gene;
- expression of the extraneous Epo gene rescues the mouse from lethality by a shortage of Epo or anemia associated therewith before birth;
- expression of the extraneous Epo gene in the kidney is suppressed after birth;
- and
- the mouse was generated from a fertilized egg deposited under accession number FERM BP-11347.
6 more elements
Dependencies are inferred from the claim text.