Updating landmarks to improve coregistration as regions of interest are corrected

A method comprising:

• displaying a first digital image of a first tissue slice on a graphical user interface such that an area of the first digital image is enclosed by a frame, wherein the displayed portion of the first digital image is larger than the frame;
• displaying a portion of a second digital image of a second tissue slice on the graphical user interface, wherein the first tissue slice and the second tissue slice are both z slices taken from a tissue sample at corresponding positions in the x and y dimensions, wherein the displayed portion of the second digital image has a shape of the frame, wherein the area of the first digital image that is enclosed by the frame corresponds to the displayed portion of the second digital image, wherein the area of the first digital image that is enclosed by the frame is coarsely coregistered with the displayed portion of the second digital image;
• positioning an outline of a region of interest on the first digital image, wherein the outline was manually generated on the second digital image, wherein a first spatial relationship between the outline on the first digital image and a first landmark on the first digital image corresponds to a second spatial relationship between the outline on the second digital image and a second landmark on the second digital image;
• manually moving a contour point on the outline on the first digital image to form a corrected outline of the region of interest on the first digital image;
• displaying the corrected outline on the first digital image on the graphical user interface;
• and
• based on how the contour point was manually moved, moving the first landmark on the first digital image such that the first spatial relationship between the corrected outline on the first digital image and the first landmark corresponds to the second spatial relationship between the outline on the second digital image and the second landmark on the second digital image.

A method comprising:

• displaying a first digital image of a first tissue slice on a graphical user interface;
• displaying a second digital image of a second tissue slice on the graphical user interface, wherein the first tissue slice is stained with a first biomarker and the second tissue slice is stained with a second biomarker, wherein the first digital image is coarsely coregistered with the second digital image using a first landmark on the first digital image and a second landmark on the second digital image;
• positioning an outline over a second region of interest on the second digital image using the second landmark, wherein the outline was manually drawn over a first region of interest on the first digital image;
• manually moving a first contour point of the outline on the second digital image to form a corrected outline;
• displaying the corrected outline over the second region of interest on the graphical user interface;
• and
• moving the second landmark based on how the first contour point was manually moved, wherein the first digital image is more finely coregistered with the second digital image after the second landmark is moved.