Optimized detector readout for biosensor
AssigneeKoninklijke Philips N.V.
InventorsJosephus Arnoldus Henricus Maria Kahlman, Bart Michiels
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Abstract
The present invention provides a biosensor system comprising a light source, a cartridge adapted to be illuminated by said light source, a light detector adapted for detecting a signal originating from the cartridge, an illumination control means adapted to vary the illumination of the cartridge between at least two different states, a means for generating a first oscillation with a first frequency, and a means for generating a second oscillation with a second frequency, wherein the frame rate of the light detector is triggered by the first oscillation and the illumination control means is triggered by the second oscillation.
Claims4
A method of detecting relative darkening at a binding spot of a biosensor cartridge having a reflective surface and label particles causing an optical inhomogeneity, said method comprising the acts of:
- illuminating the cartridge by incoming light from a light source for generating an evanescent wave having a portion scattered due to presence of the label particles;
- taking a first image of the binding spot and its surrounding at a first illumination of the cartridge by the incoming light at a frame rate determined by a first oscillation having a first frequency;
- taking a second image of the binding spot and its surrounding at a second illumination of the cartridge by the incoming light at a frame rate determined by a second oscillation having a second frequency derived by dividing the first oscillation having the first frequency;
- analyzing the intensity of said first and second images at the binding spot and at its surrounding;
- and
- calculating the relative darkening of the binding spot,
- wherein the second illumination has a higher intensity than the first illumination.
Dependencies are inferred from the claim text.