Method of calibration
AssigneeSCREEN Holdings Co., Ltd.
InventorsHiroki Fujimoto, Sanzo Moriwaki
Life of this patent
Estimating the term…
Abstract
The following processes are performed to improve the accuracy of the process of estimating the volume of a cell clump from an image including the cell clump. First, the image including the cell clump is acquired, and the optical density of the cell clump in the image is measured. Cross-section information about the cell clump is acquired by observation using a confocal microscope or by physical cutting. Based on the cross-section information, the vertical height of the cell clump is determined. Thereafter, data representing a relationship between the aforementioned optical density and the height is acquired. This improves the accuracy of the process of converting the optical density into the height to thereby achieve the accurate estimation of the volume of the cell clump.
Claims9
A method of calibration for improving the accuracy of the process of estimating the volume of a cell clump from an image including the cell clump, comprising the steps of:
- a) directing light from one of a first position lying over a cell clump held in a well having a bottom portion at least which is pervious to light and a second position lying under said cell clump toward the other of the first and second positions to receive said light at an imaging device disposed in the other of the first and second positions, thereby acquiring an image including said cell clump;
- b) measuring the optical density of said cell clump in the image acquired in said step a);
- c) acquiring cross-section information about said cell clump held in said well to determine the vertical height of said cell clump, based on said cross-section information;
- and
- d) acquiring data representing a relationship between the optical density measured in said step b) and the height determined in said step c),
- wherein in said step c), pieces of cross-section information are acquired by acquiring a distribution of cells at each of a plurality of cross-sections of said cell clump, and determining said vertical height of said cell clump at each position, based on said pieces of cross-section information.
Dependencies are inferred from the claim text.