Production of conjugates
AssigneeInnova Biosciences Limited
InventorNicholas Gee
Life of this patent
Estimating the term…
Abstract
Disclosed is a method for indirectly coupling a small molecule ligand to a molecule to be labelled with the ligand, the method comprising the step of: contacting a scaffold molecule, to which is attached at least one small molecule ligand, with the molecule to be labelled, the scaffold molecule having at least one group which is reactive towards a receiver moiety present or formed in situ on the molecule to be labelled, so as to forma bond between the scaffold molecule and the molecule to be labelled, thereby indirectly coupling the small molecule ligand to the molecule to be labelled.
Claims11
A kit for use in a method of indirectly coupling a ligand to a polypeptide to be labelled with the ligand;
- the kit comprising
- a freeze-dried aliquot comprising both (a) an isolated scaffold molecule which is covalently attached to at least one said ligand and a plurality of thiol-reactive groups reactive towards a thiol group to be formed in situ on the polypeptide to be labelled;
- and (b) a thiol-generator comprising 2-iminothiolane, for in situ creation of thiol groups on the polypeptide to be labelled
- wherein said ligand comprises a molecule selected from the group consisting of biotin, avidin, fluorescent dye, chromophore, biotin attached with N-hydroxysuccinimide, biotin attached with maleimide, avidin attached with N-hydroxysuccinimide, and avidin attached with maleimide.
Dependencies are inferred from the claim text.