Method for measurement of equol in biological sample by immunoassay, kit for the measurement, and method for determination of equol production ability of subject
AssigneesOtsuka Pharmaceutical Co., Ltd., Eiken Kagaku Kabushiki Kaisha
InventorsTakayuki Minekawa, Kumiko Shindome, Katsushi Abe, Hiroshi Okuma, Chieko Ando, Yasuhiro Abiru
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Abstract
The determination method of the present invention includes steps of measuring equol in a biological sample derived from a subject who has ingested soybean isoflavone by an immunological method using S-equol as at least one antigen selected from the group consisting of the standard antigen and the labeled antigen, and determining an equol-producing ability of the subject based on the measured value of equol obtained in the above step.
Claims10
A method for determining an S-equol-producing ability of a subject, comprising the steps of
- (1) measuring S-equol in a biological sample derived from a subject who has ingested soybean isoflavone, by an immunological method comprising:
- (a) mixing said sample with labeled S-equol, contacting the resulting mixture with an antibody that binds S-equol, and detecting the amount of the labeled S-equol bound to the antibody, wherein said antibody is a diluted antiserum, and wherein when S-equol is present in said sample, the S-equol in said sample competes with the labeled S-equol for binding to said antibody;
- and
- (b) calculating the amount of S-equol in said sample, by comparing the detected amount of the labeled S-equol bound to the antibody to a standard curve,
- wherein said standard curve has been prepared by mixing labeled S-equol with different amounts of a standard, contacting the resulting mixtures with an antibody that binds S-equol, and detecting the amount of the labeled S-equol bound to the antibody,
- wherein said standard is S-equol, and wherein said standard competes with the labeled S-equol for binding to said antibody;
- and
- (2) making a determination according to (i) or (ii):
- (i) determining that said subject has an S-equol-producing ability when S-equol is detected in said sample, or determining that said subject does not have an S-equol-producing ability when S-equol is not detected in said sample;
- or
- (ii) determining that said subject has an S-equol-producing ability when S-equol is detected in said sample in an amount equal to or greater than a pre-determined cut-off value, or determining that said subject does not have an S-equol-producing ability when S-equol is not detected in said sample, or is detected in an amount less than a pre-determined cut-off value,
- wherein the biological sample is at least one member selected from the group consisting of urine, blood, and feces.
Dependencies are inferred from the claim text.