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US 9,176,119 B2Granted
Method for quantifying a cholinergic neurotoxin in a sample
AssigneeIpsen Pharma S.A.S.
InventorsJosé-Antonio Camara-Ferrer, Michael Auguet, Pierre-Etienne Chabrier De Lassauniere
Life of this patent
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Abstract
The invention relates to a method for determining the kinetics of action of a cholinergic neurotoxin as well as a method for determining the quantity of neurotoxin in a sample.
Claims9
A method for determining the concentration of botulinum neurotoxin sample comprising the following steps:
- (a) administering the botulinum neurotoxin sample to at least one Zebra fish by intraperitoneal injection;
- (b) detecting the neuromuscular activity of the botulinum neurotoxin sample,
- wherein the neuromuscular activity is slowing of mobility, reduction in motility, immobility, paralysis, or a combination thereof;
- (c) comparing the neuromuscular activity determined in step (b) to the neuromuscular activity of botulinum neurotoxin of known concentration;
- and
- (d) determining the concentration of the botulinum neurotoxin sample.
3 more elements
A method for determining the concentration of botulinum neurotoxin sample comprising:
- (a) administering the botulinum neurotoxin sample to at least one Zebra fish by intraperitoneal injection;
- (b) detecting the biological activity of the botulinum neurotoxin sample, wherein the biological activity is slowing of mobility, reduction in motility, immobility, difference in the behavior of the fish, or a combination thereof;
- (c) comparing the biological activity determined in step (b) to the biological activity of botulinum neurotoxin of known concentration;
- and
- (d) determining the concentration of the botulinum neurotoxin sample.
2 more elements
Dependencies are inferred from the claim text.