Back to results
US 9,176,116 B2Granted
Cell culture system for determining the sensitizing, allergenic and/or irritating effect of a substance
AssigneeHOT Screen GmbH
InventorManfred Schmolz
Life of this patent
Estimating the term…
Abstract
The present invention refers to a cell culture system especially for investigating the sensitizing, allergenic and/or irritating effect of substances, comprising a first and a second compartment that can communicate with each other via a permeable interlayer, whereby the first compartment has an epidermis model and the second a cell culture based on immune cells.
Claims19
A method of determining the sensitizing or allergenic effect of a substance, comprising:
- a) contacting a surface of an epidermis model contained in a first compartment of a culture apparatus with the substance;
- b) the activity of a cell culture of immune cells comprising antigen presenting cells contained in a second compartment of said apparatus;
- and
- c) determining that the substance has a sensitizing or allergenic effect if the second compartment comprises an activated immune cell,
- wherein the first compartment and second compartment are separated by a permeable interlayer and wherein the epidermis model is in communication with the cell culture of immune cells via the permeable interlayer.
2 more elements
A method of determining the sensitizing or allergenic effect of a substance, comprising:
- a) contacting a surface of a multilayered epidermis model in a first compartment of a culture apparatus with the substance;
- b) measuring the activity of a cell culture of immune cells comprising antigen presenting cells and lymphocytes contained in a second compartment of said apparatus;
- and
- c) determining that the substance has a sensitizing or allergenic effect if the second compartment comprises an activated immune cell,
- wherein the first compartment and second compartment are separated by a permeable interlayer and wherein the multilayered epidermis model is in communication with the cell culture of immune cells via the permeable interlayer.
2 more elements
A method of determining the allergenic effect of a substance, comprising:
- a) contacting a surface of a multilayered epidermis model in a first compartment of a culture apparatus with the substance, wherein the first compartment is separated from a second compartment containing a cell culture of immune cells comprising antigen presenting cells and lymphocytes by a permeable interlayer, wherein the multilayered epidermis model is in communication with the cell culture of immune cells via the permeable interlayer;
- b) contacting the surface of the multilayered epidermis model one or more additional times with the substance;
- c) measuring an oligoclonal secondary response of the cell culture of immune cells;
- and
- d) determining that the substance has an allergenic effect if the lymphocytes comprise the oligoclonal secondary response.
2 more elements
Dependencies are inferred from the claim text.