Method of optical analysis using reference cell and base plate correction and apparatus thereof

A method of analyzing a sample comprising:

• providing an analysis unit including a base plate comprising a transparent substrate and a transparent cover, the base plate including:
• a reaction portion having at least one reaction cell including a reagent and a sample between the transparent substrate and the transparent cover,
• at least one reference portion having a reference cell including the sample between the transparent substrate and the transparent cover, and
• at least one base portion which does not comprise a cell,
• wherein the reference portion, the reaction portion, and the base portion are located in the base plate and the base portion is located at a position of the base plate where the reaction cell and the reference cell are not located,
• irradiating the reaction cell with light from a light source to obtain a reaction data from an optical detector, the reaction data indicating optical characteristics of the reaction portion;
• irradiating the reference cell with light from the light source to obtain a first reference data from the optical detector, the first reference data indicating optical characteristics of the reference portion;
• irradiating the base portion with light from the light source to obtain a second reference data from the optical detector, the second reference data indicating optical characteristics of the base portion;
• detecting the emitted light from the reaction cell, the reference cell, and the base portion, the detected light respectively being light that has passed through the reaction cell, the reference cell, or the base portion;
• and
• correcting the first reference data using the second reference data to obtain a sample data, the sample data indicating optical characteristics of the sample,
• wherein the base plate and the light source are movable relative to one another, and
• wherein a respective depth of the reaction cell and the reference cell is about 100 μm.