System and method for carrying out multiple binding reactions in an array format

A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member comprising:

• (a) adsorbing a first binding member to a surface of a plurality of microspots, wherein the adsorption occurs simultaneously in all microspots;
• (b) presenting a second binding member to the first binding member simultaneously to each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots;
• (c) obtaining data indicative of a binding reaction between the first and second binding members simultaneously from each of the plurality of microspots by a biosensor detection method;
• and
• (d) processing the data so as to obtain one or more kinetic parameters of binding between the first and second binding members;
• wherein the plurality of binding reactions does not necessitate a regeneration stage, and wherein in step (a) adsorbing the first binding member to a surface at a plurality of microspots comprises:
• (a) activating the surface in the microspot by presenting thereto a chemical activating substance by:
• (i) forming a first channel around a region containing the microspot;
• (ii) introducing a solution containing the activating substance into the channel;
• and
• (iii) removing excess activating solution from the channel;
• (b) adsorbing the first binding member to the microspot;
• and
• (c) deactivating the microspot.