Method for detection of target nucleic acid

A method for detection of a target nucleic acid in a sample, comprising:

• preparing a solid phase comprising detection probes respectively having different base sequences;
• carrying out PCR on the sample to obtain chimeric DNAs each having a label, and a tag sequence complementary to each of the detection probes having been correlated to the target nucleic acid;
• bringing the chimeric DNAs into contact with the detection probes such that the chimeric DNAs and the detection probes can hybridize through the tag sequences;
• obtaining signal intensity information based on the label on the solid phase;
• and
• detecting the target nucleic acid based on the signal intensity information;
• wherein the step of PCR comprises:
• preparing a set of primers which does not include a universal primer, wherein the set of primers consists of a first primer having an identification sequence complementary to a target sequence in the target nucleic acid and a tag addition sequence complementary to the tag sequence, and a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence and the label;
• and
• carrying out PCR on the sample using a set consisting of the first primer and the second primer to synthesize the chimeric DNA having the target sequence, the tag sequence and the label.