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US 9,175,335 B2Granted
Biomolecule interaction using atomic force microscope
AssigneesPosco, Postech Foundation
InventorsJoon Won Park, Yu Jin Jung, Bong Jin Hong, Saul Tendler, Stephanie Allen
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Abstract
The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized.
Claims11
A method for determining the presence of a mismatched nucleotide between a probe nucleotide and a target nucleotide, said method comprising:
- comparing a control atomic force microscopy (AFM) binding force with a measured AFM binding force between a probe nucleotide and a target nucleotide to determine whether there is a mismatch between said probe nucleotide and said target nucleotide,
- wherein said AFM comprises a cantilever having a fixed end and a free end, wherein the free end of said cantilever comprises a tapered protrusion of surface region, and wherein said probe nucleotide is attached to said cantilever via a dendron comprising:
- branched regions, and
- a single linear region,
- and wherein a plurality of termini of said branched regions of dendron are covalently bound to the surface region of said cantilever, and said probe nucleotide is attached to the terminus of the single linear region of said dendron, wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm.
2 more elements
Dependencies are inferred from the claim text.