Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition

A composition that possesses cell-free protein synthesis activity and that comprises an independently purified initiation factor, an independently purified elongation factor, an independently purified aminoacyl-tRNA synthetase, an independently purified ribosome, an independently purified amino acid, an independently purified nucleoside triphosphate, and an independently purified tRNA,

• wherein the initiation factor, the elongation factor, the aminoacyl-tRNA synthetase, and the ribosome each were purified from a Gram-negative bacteria,
• wherein the composition has a ribosome concentration of 0.01- 50 μM,
• wherein the independently purified ribosome is a ribosome with a reduced lipopolysaccharide content, obtained by a washing step using a buffer solution comprising a surfactant,
• wherein the independently purified ribosome has a lipopolysaccharide concentration of 0.5 EU per pmol of ribosome or less, and
• wherein the composition does not comprise any release factors.