Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass
AssigneeCodexis, Inc.
InventorsLouis A. Clark, Dipnath Baidyaroy, Lorand Szabo
Life of this patent
Estimating the term…
Abstract
The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
Claims9
A method for producing glucose from cellobiose, comprising contacting cellobiose with a recombinant cell comprising a polynucleotide encoding a recombinant β-glucosidase, wherein said polynucleotide comprises SEQ ID NO:
- 9 and is operably linked to a heterologous promoter, under conditions in which said β-glucosidase is produced by the cell and said cellobiose is enzymatically converted by said β-glucosidase to glucose.
A method of converting a biomass substrate to a fermentable sugar, comprising combining a recombinant host cell with a biomass substrate, wherein the recombinant host cell comprises a polynucleotide encoding a recombinant β-glucosidase, wherein said polynucleotide comprises SEQ ID NO:
- 9 and is optionally operably linked to a heterologous promoter, under conditions suitable for the production of the fermentable sugar.
Dependencies are inferred from the claim text.