Stable bacterial cultures for producing alginates
AssigneeMarshall University Research Corporation
InventorsHongwei D. Yu, Dongru Qiu, F. Heath Damron
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Abstract
Methods for mass producing bacterial alginate, bacterial cultures for producing alginate, and pharmaceutical compositions containing bacterial alginate are contemplated.
Claims9
A biological culture comprising isolated stable mucoid Pseudomonas aeruginosa bacteria that produce alginate, wherein the bacteria are genetically modified Pseudomonas aeruginosa bacteria having increased protease activity of a ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria that is the result of transformation of the Pseudomonas aeruginosa bacteria with an exogenous DNA encoding the ClpP, ClpP2, or ClpX polypeptide to overexpress the ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria and wherein the bacteria further have at least one characteristic selected from the group consisting of:
- (i) increased positive regulator activity of a MucE polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria, wherein the increased MucE regulator activity is the result of transformation of the Pseudomonas aeruginosa bacteria with an exogenous DNA encoding the MucE polypeptide to overexpress the MucE polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria,
- (ii) decreased negative regulator activity of a MucA polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria, wherein the decreased MucA regulator activity is the result of a transposon insertion in the promoter of a mucA gene of the Pseudomonas aeruginosa bacteria, a mutation in the MucA polypeptide that results in the MucA polypeptide lacking a portion or all of a transmembrane domain, and/or a single base deletion at T180 of the mucA gene of the Pseudomonas aeruginosa bacteria, and
- (iii) lack of expression of a lipopolysaccharide do not express at least one endotoxin biosynthesis gene.
A biological culture comprising isolated stable mucoid Pseudomonas aeruginosa bacteria that produce alginate, wherein the bacteria are genetically modified Pseudomonas aeruginosa bacteria having increased protease activity of a ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria that is the result of transformation of the Pseudomonas aeruginosa bacteria with an exogenous DNA encoding the ClpP, ClpP2, or ClpX polypeptide to overexpress the ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria.
Dependencies are inferred from the claim text.