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AssigneeDyadic Nederland B.V.
InventorsPeter J. Punt, Richard Paul Burlingame, Christine M. Pynnonen, Phillip T. Olson, Jan Wery, Johannes Heinrich Visser, Mark A. Emalfarb, Jacob Visser, Jan Cornelis Verdoes
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The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
A strain of Chrysosporium lucknowense selected from the group consisting of W1L deposited at CBS under accession number 122189, W1L#100.1 deposited at CBS under accession number 122190, and a strain derived therefrom by gene disruption.
A method for isolating a genetically modified fungal strain of Chrysosporium lucknowense strain UV 18-25, wherein cellulase and protease secretion is less than 20% of endogenous cellulase and protease secretion of unmodified Chrysosporium lucknowense strain UV 18-25, comprising the steps of:
A method for isolating a genetically modified fungal strain of Chrysosporium lucknowense strain UV 18-25, wherein cellulase secretion is less than 20% of endogenous cellulase secretion of unmodified Chrysosporium lucknowense strain UV 18-25, comprising the steps of:
Dependencies are inferred from the claim text.