Compositions and methods for bacterial and genetically modified microorganism production of chondroitin
AssigneeSeikagaku Corporation
InventorsDaniel H. Doherty, Craig A. Weaver, Kentaro Miyamoto, Toshikazu Minamisawa
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Abstract
The invention relates to the field of recombinant DNA technology for the production of chondroitin, including the production of chondroitin sulfate via a combination of recombinant bacterial fermentation and post-fermentation sulfation.
Claims26
A construct comprising a kfoA, kfoC, and kfoF gene, wherein the construct does not contain a functional gene of one or more of kfoD, orf3(kfoI), kfoE, or orf1(kfoH), and wherein the construct is suitable for producing chondroitin in a non-pathogenic bacterial host cell.
A method for producing a bacterial cell that is capable of producing non-fructosylated chondroitin, wherein the cell comprises a kfoA gene, a kfoC gene, and a kfoF gene, the method comprising inactivating a gene in the cell selected from the group consisting of:
- kfoD, orf3(kfoI), kfoE, orf1(kfoH), and combinations thereof.
A recombinant bacterial cell comprising a kfoA gene, a kfoC gene, and a kfoF gene, wherein the cell does not comprise a functional gene of one or more of kfoD, orf3(kfoI), kfoE, or orf1(kfoH), and wherein the cell is capable of producing chondroitin.
A genetically modified microorganism comprising a kfoA gene, a kfoC gene, and a kfoF gene, wherein the microorganism has been genetically modified to delete or inactivate a gene of one or more of kfoD, orf3(kfoI), kfoE, or orf1(kfoH), and wherein the microorganism is capable of producing chondroitin.
Dependencies are inferred from the claim text.