Use polysaccharides for promotion of enzymatic activity
AssigneeGenzyme Corporation
InventorsFrank Riske, Michael Hayes, Gary Lazarus
Life of this patent
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Abstract
This disclosure provides methods and compositions for the promotion of enzymatic activity of Target Enzymes, including but not limited to oligosaccharide/polysaccharide enzymes, protein enzymes, polynucleotide enzymes. The methods involve use of a non-naturally occurring polysaccharide (including but not limited HES) for promoting the enzymatic activity of an enzyme in liquid milieu, wherein the concentration of the polysaccharide in the composition comprising the Target Enzyme is from about 0.01% to about 55% w/v.
Claims23
A method of promoting enzymatic activity of a target enzyme comprising:
- (a) combining a target enzyme wherein the target enzyme is one or more oligosaccharide/polysaccharide enzymes, lysosomal hydrolases or a combination thereof with a hydroxyalkyl starch and a substrate of the target enzyme, thereby producing a composition, wherein the composition comprises about 0.01% to about 55% w/v of the hydroxyalkyl starch wherein the hydroxyalkyl starch is not conjugated to the target enzyme or the substrate;
- and
- (b) maintaining the composition under conditions in which enzymatic alteration of the substrate by the target enzyme occurs, thereby promoting the enzymatic activity of the target enzyme.
A method of promoting the enzymatic activity of sialidase, β-galactosidase, and β-hexosaminidase comprising:
- (a) combining the sialidase, β-galactosidase, and β-hexosaminidase with a substrate of the sialidase, β-galactosidase and β-hexosaminidase and hydroxyethyl starch (HES), thereby producing a composition, wherein the composition comprises between about 1% and about 12% w/v of the HES wherein the HES is not conjugated to the sialidase, β-galactosidase, and β-hexosaminidase or the substrate;
- and
- (b) maintaining the composition under conditions in which the sialidase, β-galactosidase, and β-hexosaminidase modify the substrate, thereby promoting the enzymatic activity of sialidase, β-galactosidase and β-hexosaminidase.
A method of promoting enzymatic activity of a target enzyme comprising:
- (a) combining a target enzyme wherein the target enzyme is one or more oligosaccharide/polysaccharide enzymes, lysosomal hydrolases or a combination thereof with a hydroxyalkyl starch and a substrate of the target enzyme, thereby producing a composition, wherein the composition comprises about 0.01% to about 55% w/L sv of the hydroxyalkyl starch wherein the hydroxyalkyl starch is not conjugated to the target enzyme or the substrate;
- and
- (b) maintaining the composition under conditions in which enzymatic alteration of the substrate by the target enzyme occurs, thereby promoting the enzymatic activity of the target enzyme.
Dependencies are inferred from the claim text.