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US 9,175,281 B2Granted
Process for the purification of tissue plasminogen activator
AssigneeCadila Healthcare Limited
InventorsSanjay Bandyopadhyay, Sanjeev Kumar Mendiratta, Sushobhan Bandyopadhyay
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Abstract
The present invention provides process of purification of tissue plasminogen activator (tPA) from a crude mixture or a partially purified mixture containing tPA protein and impurities by using hydrophobic interaction column chromatography, optionally in combination with ion exchange column chromatography.
Claims25
A process for the purification of a tissue plasminogen activator protein comprising:
- a) loading a mixture of a tissue plasminogen activator protein with impurities on to a suitable column matrix;
- b) eluting the impurities from the column matrix with a suitable organic solvent at appropriate concentration;
- c) eluting the desired form of the tissue plasminogen activator protein from the column matrix with a suitable organic solvent at appropriate concentration;
- d) obtaining the tissue plasminogen activator protein in its desired form;
- and
- e) optionally, an ion exchange column step is used before or after using the suitable column matrix;
- wherein the suitable organic solvent is ethanol, methanol, or isopropyl alcohol, and
- wherein the suitable column matrix is hydrophobic interaction chromatography.
5 more elements
Dependencies are inferred from the claim text.