Polypeptides having endoglucanase activity and polynucleotides encoding same
AssigneeNovozymes, Inc.
InventorNikolaj Spodsberg
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Abstract
The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims36
A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heteroloqous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having endoglucanase activity comprises:
- an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, or at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 8.
A process for degrading a cellulosic material, said process comprising:
- (i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;
- and
- (ii) recovering the degraded material;
- wherein the polypeptide having endoglucanase activity comprises at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, or at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 8.
A process for producing a fermentation product, said process comprising:
- (a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation;
- wherein the polypeptide having endoglucanase activity comprises at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, or at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 8.
A nucleic acid construct or expression vector comprising a gene encoding a protein,
- wherein the gene is operably linked to a polynucleotide that encodes a signal peptide comprising or consisting of amino acids 1 to 22 of SEQ ID NO:
- 2, amino acids 1 to 23 of SEQ ID NO:
- 4, amino acids 1 to 20 of SEQ ID NO:
- 6, or amino acids 1 to 19 of SEQ ID NO:
- 8, and
- wherein the gene is foreign to the polynucleotide encoding the signal peptide.
An isolated recombinant host cell transformed with a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is heterologous to the recombinant host cell, and wherein the polypeptide having endoglucanase activity comprises at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, or at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.