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US 9,175,270 B2Granted
Method of modifying a yeast cell for the production of ethanol
AssigneeDanisco US Inc.
InventorsElke Nevoigt, Stephane Guillouet, Carine Bideaux, Sandrine Alfenore, Georg Hubmann
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Abstract
The invention relates to a method of modifying a yeast cell for the production of ethanol. According to some embodiments of the invention, the activity of the Gpd1 protein and/or the Gpd2 protein is reduced.
Claims7
A method of modifying a yeast cell for the production of ethanol, the method comprising:
- reducing, but not eliminating, glycerol-3-phosphate dehydrogenase 1, Gpd1, protein activity in the yeast cell and eliminating glycerol-3-phosphate dehydrogenase 2, Gpd2, protein activity in the yeast cell;
- or
- eliminating Gpd1 protein activity in the yeast cell and reducing, but not eliminating, Gpd2 protein activity in the yeast cell;
- or
- reducing, but not eliminating, both Gpd1 and Gpd2 protein activity in the yeast cell,
- wherein the reduction of Gpd1 protein activity and/or Gpd2 protein activity is achieved by reducing the expression of the GPD1 gene and/or the GPD2 gene by at least 50% compared to the expression of the GPD1 gene and/or the GPD2 gene in the unmodified wild type yeast cell and wherein the modified yeast cell has equivalent growth to that of an unmodified yeast cell under oxygen-limited conditions and an increased yield of ethanol.
3 more elements
Dependencies are inferred from the claim text.