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US 9,175,268 B2Granted
Methods for the production of iPS cells
AssigneeCellular Dynamics International, Inc.
InventorAmanda Mack
Life of this patent
Estimating the term…
Abstract
Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating induced pluripotent stem cells using reporter genes are described. Furthermore, the invention provides novel reprogramming vectors employing reporter genes.
Claims22
A reprogramming vector comprising a polycistronic expression cassette, wherein the polycistronic expression cassette comprises a transcriptional regulatory element;
- and a first and second coding sequence operably linked to said transcriptional regulatory element, wherein said first coding sequence encodes a first reprogramming factor and said second coding sequence encodes a second reprogramming factor, wherein the reprogramming vector is an Epstein-Barr Virus (EBV)-based vector, and further wherein the first and second reprogramming factor are selected from the group consisting of Sox, Oct, Nanog, Lin28, Klf4, and c-Myc.
Dependencies are inferred from the claim text.