Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity
AssigneeExxonMobil Research and Engineering Company
InventorsYou Chen, Thomas E. Wall, Stanley Bower, Robert Christopher Brown, Jennifer Coppersmith
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Abstract
The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.
Claims24
A recombinant microorganism comprising a non-native nucleic acid molecules encoding a polypeptide having lipolytic activity, wherein the polypeptide comprises an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:40, and wherein the recombinant microorganism has attenuated expression of
- at least one endogenous gene selected from the group consisting of an acyl-CoA synthetase gene and an acyl-ACP synthetase gene as compared to a corresponding wild-type microorganism, and wherein
- the recombinant microorganism produces at least one free fatty acid or at least one fatty acid derivative.
An expression cassette comprising a nucleic acid molecule encoding a polypeptide having lipolytic activity, wherein the polypeptide comprises an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:40, and wherein the nucleic acid molecule encoding a polypeptide having lipolytic activity is operably linked to a heterologous promoter.
A recombinant microorganism comprising a non-native nucleic acid molecule encoding a polypeptide having lipolytic activity, wherein the polypeptide comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:38 and SEQ ID NO:47, and wherein the recombinant microorganism has attenuated expression of
- at least one endogenous gene selected from the group consisting of an acyl-CoA synthetase gene and an acyl-ACP synthetase gene as compared to a corresponding wild-type microorganism, and wherein
- the recombinant microorganism produces at least one free fatty acid or at least one fatty acid derivative.
An expression cassette comprising a nucleic acid molecule encoding a polypeptide having lipolytic activity, wherein the polypeptide comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:38 and SEQ ID NO:47, and wherein the nucleic acid molecule encoding a polypeptide having lipolytic activity is operably linked to a heterologous promoter.
A recombinant microorganism comprising a non-native nucleic acid molecule encoding a polypeptide having lipase activity, wherein the polypeptide comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, and SEQ ID NO:6, and wherein the recombinant microorganism has attenuated expression of
- at least one endogenous gene selected from the group consisting of an acyl-CoA synthetase gene and an acyl-ACP synthetase gene as compared to a corresponding wild-type microorganism, and wherein
- the recombinant microorganism produces at least one free fatty acid or at least one fatty acid derivative.
Dependencies are inferred from the claim text.