US 9,175,035 B2 · Oligopeptide sequence specifically bonding to phenylboronic acid group

Abstract

An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.

Claims24

Dependent claims

A method for immobilizing a molecule, comprising the steps of:

attaching a label peptide sequence comprising two or more amino acid residues of hydroxyl group-containing amino acids, and one to three amino acid residues selected from the group consisting of amino acid residues of basic amino acids and tryptophan residue to a molecule to form a labeled molecule;
providing a molecule having a phenylboronic acid group with one or more functional groups bound to a solid support or crosslinked by a bridging molecule to form an immobilization substrate;
and
bringing the molecule having a phenylboronic acid group into contact with the labeled molecule, to bind and immobilize the labeled molecule by the molecule having a phenylboronic acid group.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

13/982,755Filed 30 Jan 2012

Priority

JP 2011-01816331 Jan 2011

Published

US 2013/0344590 A126 Dec 2013

Granted

3 Nov 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C07K 1/22
By chromatography: affinity chromatography or related techniques based upon selective absorption processes
C07K 17/06
Peptides being immobilised on, or in, an organic carrier: attached to the carrier via a bridging agent
C07K 17/08
Peptides being immobilised on, or in, an organic carrier: the carrier being a synthetic polymer
C07K 17/14
Carrier-bound or immobilised peptides ; Preparation thereof: peptides being immobilised on, or in, an inorganic carrier

Oligopeptide sequence specifically bonding to phenylboronic acid group

Life of this patent

Oligopeptide sequence specifically bonding to phenylboronic acid group

Life of this patent

Abstract

Claims24