Method for purifying or detecting a target protein
AssigneeLaboratoire Francais Du Fractionnement Et Des Biotechnologies
InventorsLaurent Siret, Abdessatar Chtourou, Frédéric Dhainaut, Gérald Perret
Life of this patent
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Abstract
A method for purifying or detecting a target protein present in a solution, includes, before carrying out the actual detection or purification step, a step of contacting the solution with an aptamer binding specifically to the target protein, where the aptamer does not bind to a protein homologous to the target protein that could also be present in the solution.
Claims18
A method for purifying a human factor VII present in a biological solution, obtained from a non-human transgenic animal, said method comprising:
- contacting said biological solution with a single stranded DNA aptamer immobilized onto a solid support through a spacer, said immobilized aptamer binding specifically to said human factor VII but not to any protein homologous to said human factor VII;
- and then
- purifying the human factor VII,
- wherein said biological solution is a non-human transgenic mammal's milk, a non-human transgenic mammal's milk-derived product, or a non-human transgenic mammal's clarified milk.
A method for purifying specifically a human factor VII from a biological solution, obtained from a non-human transgenic animal, which contains said human factor VII and which may contain a protein homologous to said human factor VII, comprising the steps of:
- a) providing a solid support comprising a single stranded DNA aptamer immobilized onto said solid support through a spacer, wherein said immobilized aptamer does specifically bind to said human factor VII but not to a protein homologous to said human factor VII,
- b) contacting said solid support with said biological solution, and
- c) recovering the human factor VII bound to said aptamer,
- wherein said biological solution is a non-human transgenic mammal's milk, a non-human transgenic mammal's milk-derived product, or a non-human transgenic mammal's clarified milk.
A method for detecting specifically a human factor VII from a biological solution, obtained from a non-human transgenic animal, which contains said human factor VII and which may contain a protein homologous to said human factor VII, comprising the steps of:
- a) contacting said biological solution with a single stranded DNA aptamer immobilized onto a solid support through a spacer, said immobilized aptamer binding specifically to said human factor VII but not to said protein homologous to said human factor VII, and
- b) detecting complexes formed between said aptamer and said human factor VII,
- wherein the aptamer comprises a nucleotide sequence at least 80% identical to SEO ID NO:1.
A method for purifying a human factor VII present in a biological solution, obtained from a non-human transgenic animal, said method comprising:
- contacting said biological solution with a single stranded DNA aptamer immobilized onto a solid support through a spacer, said immobilized aptamer binding specifically to said human factor VII but not to any protein homologous to said human factor VII, wherein the aptamer comprises a nucleotide sequence at least 80% identical to SEQ ID NO:1;
- and then
- purifying the human factor VII.
A method for purifying specifically a human factor VII from a biological solution, obtained from a non-human transgenic animal, which contains said human factor VII and which may contain a protein homologous to said human factor VII, comprising the steps of:
- a) providing a solid support comprising a single stranded DNA aptamer immobilized onto said solid support through a spacer, wherein said immobilized aptamer does specifically bind to said human factor VII but not to a protein homologous to said human factor VII, and wherein the aptamer comprises a nucleotide sequence at least 80% identical to SEQ ID NO:1,
- b) contacting said solid support with said biological solution, and
- c) recovering the human factor VII bound to said aptamer.
Dependencies are inferred from the claim text.