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US 9,174,217 B2Granted
Device and method of amplifying nucleic acids by using oil layer comprising exothermal particles
AssigneeSamsung Electronics Co., Ltd.
InventorJong-myeon Park
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Abstract
The disclosure describes example devices and methods for amplifying nucleic acids by using an oil layer including exothermal particles, forming an emulsion using the oil layer including exothermal particles, and performing a nucleic acids amplification reaction in the emulsion. The nucleic acids amplification reaction may be effectively performed even with a small amount of a sample by maximizing nucleic acids amplification efficiency, reducing reaction time, and improving controls to be convenient.
Claims9
A device for amplifying nucleic acids comprising:
- a reaction vessel, wherein the vessel comprises a first aqueous solution comprising template nucleic acids each of which comprises a target site to be amplified and a reagent for amplifying the target site;
- an oil layer surrounding and sealing the first aqueous solution, wherein the oil layer comprises exothermal particles consisting of iron oxide and, optionally, a surfactant;
- and
- a second aqueous solution surrounding the oil layer.
1 more element
A method of amplifying nucleic acids, the method comprising:
- mixing template nucleic acids comprising a target site to be amplified, a first aqueous solution comprising a reagent for amplifying the target site, and an oil, wherein the oil comprises exothermal particles consisting of iron oxide and, optionally, a surfactant;
- forming a first emulsion in which the oil surrounds the first aqueous solution;
- forming a second emulsion in which a second aqueous solution surrounds the first emulsion by adding the second aqueous solution to the first emulsion;
- and
- performing a nucleic acid amplification reaction by heating the exothermal particles.
2 more elements
Dependencies are inferred from the claim text.