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US 9,174,212 B2Granted
System and method for particle filtration
AssigneeCytovera Inc.
InventorLotien R. Huang
Life of this patent
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Abstract
Embodiments of the present disclosure feature a filtration system comprising a filtration module for particle filtration and methods of using the device for the isolation of particles (e.g., viable cells). Advantageously, embodiments of the device provide for the high throughput filtration of large volumes of sample while preserving cell viability and providing high yields.
Claims44
A filtration device including at least one filtration unit, the at least one filtration unit comprising:
- a first sidewall;
- a second sidewall;
- a first fluid inlet defined between the first sidewall and the second sidewall, the first fluid inlet configured to receive a feed comprising a particle population and a fluid;
- a first row of pillars;
- a first plurality of pores defined by spacings between adjacent pillars in the first row of pillars;
- a first flow chamber defined between the first row of pillars and the second sidewall and including a first flow chamber inlet and a first retentate outlet, the first flow chamber configured to direct a first retentate including at least a portion of the particle population to the first retentate outlet;
- a second flow chamber defined between the first row of pillars and the first sidewall and including a first filtrate outlet, the second flow chamber configured to direct a first filtrate including at least a portion of the fluid to the first filtrate outlet;
- wherein a distance between the first row of pillars and the first sidewall proximate the first flow chamber inlet is smaller than a distance between the first row of pillars and the first sidewall proximate the first retentate outlet;
- a second row of pillars;
- a second plurality of pores defined by spacings between adjacent pillars in the second row of pillars;
- and
- a third flow chamber defined between the second row of pillars and the second sidewall, the third flow chamber including a third flow chamber inlet configured to receive the first retentate and the first filtrate and a second retentate outlet, the third flow chamber being configured to direct at least a portion of the particle population to the second retentate outlet, a distance between the second row of pillars and the first sidewall proximate the third flow chamber inlet being smaller than a distance between the third row of pillars and the first sidewall proximate the second retentate outlet.
9 more elements
A filtration device including at least one filtration unit, the at least one filtration unit comprising:
- a first sidewall;
- a second sidewall;
- a first fluid inlet defined between the first sidewall and the second sidewall, the first fluid inlet configured to receive a feed comprising a particle population and a fluid;
- a first row of pillars including an upstream end and a downstream end;
- a first plurality of pores defined by spacings between adjacent pillars in the first row of pillars;
- wherein a distance between the first row of pillars and the first sidewall proximate the upstream end of the first row of pillars is smaller than a distance between the first row of pillars and the first sidewall proximate the downstream end of the first row of pillars, and a first filtrate flow chamber free of pillars is defined extending between the first row of pillars and the first sidewall;
- a second row of pillars disposed downstream of the upstream end of the first row of pillars;
- and
- a second plurality of pores defined by spacings between adjacent pillars in the second row of pillars;
- wherein a distance between the second row of pillars and the second sidewall proximate the upstream end of the second row of pillars is smaller than a distance between the second row of pillars and the second sidewall proximate the downstream end of the second row of pillars, and a second filtrate flow chamber free of pillars is defined extending between the second row of pillars and the second sidewall.
7 more elements
A filtration system comprising:
- a fluid line configured to receive a liquid suspension of cells comprising a target cell population suspended in a fluid;
- a first collection chamber;
- a second collection chamber;
- and
- at least one cartridge comprising:
- a cartridge inlet,
- a first cartridge outlet fluidically connected to the first collection chamber,
- a second cartridge outlet fluidically connected to the second collection chamber, and
- at least one filtration unit, the at least one filtration unit having:
- a filtration unit inlet fluidically connected to the cartridge inlet,
- a first filtration unit outlet fluidically connected to the first cartridge outlet,
- a second filtration unit outlet fluidically connected to the second cartridge outlet, and
- a flow chamber configured to separate the target cell population from at least a portion of the fluid, to direct the target cell population to the first filtration unit outlet, and to direct the at least a portion of the fluid to the second filtration unit outlet, the flow chamber including:
- a first sidewall,
- a second sidewall,
- a flow chamber inlet,
- a flow chamber outlet including a filtrate outlet and a retentate outlet,
- a first row of pillars,
- a first plurality of pores defined by spacings between adjacent pillars in the first row of pillars, a distance between the first row of pillars and the first sidewall proximate the flow chamber inlet being smaller than a distance between the first row of pillars and the first sidewall proximate the flow chamber outlet,
- a second row of pillars, a distance between the second row of pillars and the second sidewall proximate the flow chamber inlet being smaller than a distance between the second row of pillars and the second sidewall proximate the flow chamber outlet,
- a downstream end of the first row of pillars and a downstream end of the second row of pillars defining the retentate outlet, the retentate outlet being in fluid communication with the first filtration unit outlet,
- a first filtrate chamber free of pillars extending from the first row of pillars to the first sidewall and occupying the volume between the first row of pillars to the first sidewall, and
- a second filtrate chamber free of pillars extending from the second row of pillars to the second sidewall and occupying the volume between the second row of pillars to the second sidewall,
- the first collection chamber, the second collection chamber, and the at least one cartridge forming a closed system.
21 more elements
Dependencies are inferred from the claim text.