Water-dispersable nanoparticles
AssigneeLife Technologies Corporation
InventorsEric Tulsky, Kari Haley, Imad Naasani, John Mauro, Roman Rozhkov, Joseph A. Treadway
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Abstract
Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.
Claims12
A population of water-dispersible nanoparticles, comprising:
- a plurality of nanoparticles,
- wherein each nanoparticle includes a nanocrystal core including a first semiconductor material, a shell including a second semiconductor material, and an outer layer that imparts hydrophilic properties to the nanoparticle,
- wherein the outer layer is comprised of a plurality of hydrophilic peptide ligands and a plurality of phosphonic acid ligands each with at least one linking group for attachment to a surface of the shell,
- wherein the ratio of the plurality of hydrophilic ligands to the plurality of phosphonic acid ligands is at least about 0.1:1.
A population of water-dispersible nanoparticles, comprising:
- a plurality of nanoparticles, wherein each nanoparticle includes a nanocrystal core including a first semiconductor material, a shell including a second semiconductor material, and an outer layer that imparts hydrophilic properties to the nanoparticle,
- wherein the outer layer is comprised of a plurality of tridentate thiol ligands and a plurality of phosphonic acid ligands each with at least one linking group for attachment to a surface of the shell,
- wherein the ratio of the plurality of tridentate thiol ligands to the plurality of phosphonic acid ligands is at least about 0.5:1, wherein the tridentate thiol ligand is a compound of Formula I, II, III, IV, V, or VI:
- wherein R1, R2, and R3, when taken alone, are independently H, halo, hydroxyl, (—(C═O)—C1-C22, —(C═O)CF3,) alkanoyl, C1-C22 alkyl, C1-C22 heteroalkyl, ((CO)OC1-C22) alkylcarbonato, or (—(CO)NH(C1-C20) or —(CO)N(C1-C20)2) alkylcarbamoyl;
- R4, and R5, when taken alone, are independently H, C1-C20 alkyl, or C6-C18 aryl;
- and
- R6 is H or a polyethylene glycol moiety.
Dependencies are inferred from the claim text.