Image guided protocol for cell generation
AssigneeDRVision Technologies LLC
InventorShih-Jong James Lee
Life of this patent
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Abstract
Computerized image guided control method uses image quantification to dynamically monitor and control the cell generation process. The method acquires images during the cell generation steps and uses computer image analysis to automatically quantify the results of the cell generation steps. In one embodiment, the quantified results from the image analysis are used to determine the readiness of the cell generation step. In another embodiment, a remedial recovery sub-step for the cell generation step is also guided by the imaging results. In yet another embodiment, when a cell generation step is determined to have failed, the cell generation process is early terminated based on image guided decision.
Claims29
A computerized image guided step readiness control method for cell generation having a plurality of generation steps, said method comprising the steps of:
- a) providing at least one source cell and a factor 1 into a cell culture for a cell generation process;
- b) performing generation step 1 in the cell culture by inducing-the factor 1 to the at least one source cell to obtain a step 1 output;
- c) acquiring a step 1 image into a computer from the step 1 output and performing a computerized image guided decision for the generation step 1 by performing computer image analysis of the step 1 image on the computer to obtain a computerized step 1 decision output;
- d) if the computerized step 1 decision output is “No-Go”, waiting for a waiting period;
- then repeating step c) if a maximum number of allowable decision attempts is not reached, or exiting cell generation if the maximum number of allowable decision attempts is reached;
- and
- e) if the computerized step 1 decision output is “Go”, performing a next generation step in the cell culture by inducing a next factor to the step 1 output to obtain a next step output.
The computerized image guided step readiness control method of claim wherein the step 1 image is acquired at a plurality of time points during generation step 1.
A computerized image guided step recovery control method for cell generation having a plurality of generation steps, said method comprising the steps of:
- a) providing at least one source cell and a factor 1 into a cell culture for a cell generation process;
- b) performing generation step 1 in the cell culture by inducing the factor 1 to the at least one source cell obtain a step 1 output;
- c) acquiring a step 1 image into a computer from the step 1 output and performing a computerized image guided decision for the generation step 1 by performing computer image analysis of the step 1 image on the computer to obtain a computerized step 1 decision output;
- d) if the computerized step 1 decision output is “No-Go Recovery”, performing recovery process for generation step 1 in the cell generation system:
- then repeating step c)if a maximum number of allowable decision attempts is not reached, or exiting cell generation if the maximum number of allowable decision attempts is reached;
- e) if the computerized step 1 decision output is “No-Go Wait”, waiting for a waiting period;
- then repeating step c) if a maximum number of allowable decision attempts is not reached, or exiting cell generation if the maximum number of allowable decision attempts is reached;
- and
- f) if the computerized step 1 decision output is “Go”, performing a next generation step in the cell culture by inducing a next factor to the step 1 output to obtain a next step output.
A computerized image guided step rejection control method for cell generation having a plurality of generation steps, said method comprising the steps of:
- a) providing at least one source cell and a factor 1 into a cell culture for a cell generation process:
- b) performing generation step 1 in the cell culture by inducing the factor 1 to the at least one source cell to obtain a step 1 output;
- c) acquiring a step 1 image into a computer from the step 1 output and performing a computerized image guided decision for the generation step 1 by performing computer image analysis of the step 1 image on the computer to obtain a computerized step 1 decision output;
- d) exiting cell generation if the computerized step 1 decision output is “No-Go Rejection”;
- e) if the computerized step 1 decision output is “No-Go Wait”, waiting for a waiting period;
- then repeating step c) if a maximum number of allowable decision attempts is not reached, or exiting cell generation if the maximum number of allowable decision attempts is reached;
- and
- f) if the computerized step 1 decision output is “Go”, performing a next generation step by inducing a next factor to the step 1 output in the cell culture to obtain a next step output.
Dependencies are inferred from the claim text.