Treatment of membrane bound transcription factor peptidase, site 1 (MBTPS1) related diseases by inhibition of natural antisense transcript to MBTPS1
AssigneeCuRNA, Inc.
InventorsJoseph Collard, Olga Khorkova Sherman
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Abstract
The present invention relates to antisense oligonuclotides that modulate the expression of and/or function of Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1), in particular, by targeting natural antisense polynucleotides of Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of MBTPS1.
Claims15
A method of upregulating a function of and/or the expression of a Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) poly in patient cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one antisense oligonucleotide 15 to 30 nucleotides in length wherein said at least one oligonucleotide is specific for and specifically hybridizes to a natural antisense polynucleotide of the MBTPS1 gene and has at least 80% sequence identity to a reverse complement of a polynucleotide comprising 15 to 30 nucleotides within nucleotides 1 to 1240 of SEQ ID NO:
- 2;
- thereby upregulating a function of and/or the expression of the Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide in patient cells or tissues in vivo or in vitro.
A method of upregulating a function of and/or the expression of a Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide in patient cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one antisense oligonucleotide 15 to 30 nucleotides in length wherein said at least one oligonucleotide is specific for and specifically hybridizes to a natural antisense polynucleotide of the MBTPS1 gene and has at least 90% sequence identity to a reverse complement of a natural antisense of a Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide;
- thereby upregulating a function of and/or the expression of the Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide in patient cells or tissues in vivo or in vitro.
A method of upregulating a function of and/or the expression of a Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide in patient cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one antisense oligonucleotide of 15 to 30 nucleotides in length that specifically targets and specifically hybridizes to a region of a natural antisense polynucleotide of the Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide wherein said natural antisense polynucleotide comprises SEQ ID NO:
- 2;
- thereby upregulating a function of and/or the expression of the Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide in patient cells or tissues in vivo or in vitro.
A method of upregulating a function of and/or the expression of Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) in mammalian cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one antisense oligonucleotide of about 15 to 30 nucleotides in length specific for and that specifically hybridizes to a natural antisense strand of a Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide wherein said at least one antisense oligonucleotide has at least 80% identity to 15-30 nucleotides of at least one nucleic acid sequence set forth as SEQ ID NO:
- 1 or an RNA polynucleotide transcribed from the MBTPS1 gene;
- and, upregulating the function and/or expression of the Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) in mammalian cells or tissues in vivo or in vitro.
A method of preventing or treating a disease associated with at least one Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide and/or at least one encoded product thereof, comprising:
- administering to a patient a therapeutically effective dose of at least one antisense oligonucleotide of 15 to 30 nucleotides in length that specifically hybridizes to a natural antisense polynucleotide of said at least one Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide wherein said natural antisense polynucleotide comprises SEQ ID NO:
- 2 and upregulates expression of said at least one Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide;
- thereby preventing or treating the disease associated with the at least one Membrane Bound Transcription Factor Peptidase, site 1 (MBTPS1) polynucleotide and/or at least one encoded product thereof.
Dependencies are inferred from the claim text.