Thermostable phytase variants
AssigneeNovozymes A/S
InventorsLeonardo De Maria, Lars Kobberoee Skov, Michael Skjoet
Life of this patent
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Abstract
The present invention relates to a method for producing phytase variants has at least 70% identity to a phytase derived from Buttiauxella and comprises at least one additional disulfide bond as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
Claims32
A variant phytase which
- a) has at least 80% identity to amino acids 1-413 of SEQ ID NO:2, amino acids 1-413 of SEQ ID NO:4, and/or amino acids 1-413 of SEQ ID NO:6 when aligned to the respective amino acid sequence using the Needle program with the BLOSUM62 substitution matrix, a gap opening penalty of 10.0, and a gap extension penalty of 0.5;
- and
- b) comprises a disulfide bridge at one or more position pairs selected from the group consisting of:
- A) 143C/201c, B) 33C/179C, C) 54C/101C, D) 93C/48C, E) 33C/178C, F) 61C/102C, and G) 164C/251C.
Dependencies are inferred from the claim text.