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US 9,173,371 B2Granted
Tobacco compositions and methods of making
AssigneeU.S. Smokeless Tobacco Company LLC
InventorsMark T. Nielsen, Marcos F. Lusso, Francis Antoine
Life of this patent
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Abstract
The present disclosure describes tobacco varieties having reduced levels of DVTs, methods of making such varieties, and tobacco compositions including such varieties.
Claims11
A method of producing a plant variety, comprising the steps of:
- crossing first tobacco plants with second tobacco plants to produce progeny plants, said first tobacco plants having less than 20 μg DVTs/cm2 green leaf tissue and said second tobacco plants having greater than 20 μg DVTs/cm2 green leaf tissue;
- allowing said progeny plants to self-pollinate for at least one generation;
- selecting, in at least one generation, for a) plants having less than 20 μg DVTs/cm2 green leaf tissue and
- b) plants, wherein cured tobacco from said plants has reduced levels of DVTs relative to cured tobacco from plants of the variety designated KY14.
1 more element
A method of producing a plant variety, comprising the steps of:
- mutagenizing tobacco plant tissue;
- propagating plants derived from said mutagenized plant tissue by self-pollination for at least one generation;
- selecting, in at least one generation, for a) plants having less than 20 μg DVTs/cm2 green leaf tissue and
- b) plants, wherein cured tobacco from said plants has reduced levels of DVTs relative to cured tobacco from plants of the variety designated KY14.
1 more element
A method of producing a plant variety, said method comprising the steps of:
- crossing first tobacco plants with second tobacco plants to produce progeny plants, wherein tissue from said first tobacco plants has significantly reduced bitterness in a sensory panel evaluation relative to tissue from plants of the variety designated KY14 and wherein tissue from said second tobacco plants does not have significantly reduced bitterness in a sensory panel evaluation relative to tissue from plants of the variety designated KY14;
- allowing said progeny plants to self-pollinate for at least one generation;
- and
- selecting, in at least one generation, for a) plants having less than 20 μg DVTs/cm2 green leaf tissue and
- b) plants, wherein cured tobacco from said plants has reduced levels of DVTs relative to cured tobacco from plants of the variety designated KY14.
2 more elements
A method of producing a plant variety, said method comprising the steps of:
- crossing first tobacco plants with second tobacco plants to produce progeny plants, wherein cured tobacco from said first tobacco plants has reduced levels of DVTs relative to cured tobacco from plants of the variety designated KY14 and wherein cured tobacco from said second tobacco plants does not have reduced DVTs relative to cured tobacco from plants of the variety designated KY14;
- allowing said progeny plants to self-pollinate for at least one generation;
- and
- selecting, in at least one generation, for a) plants having less than 20 μg DVTs/cm2 green leaf tissue and
- b) plants, wherein cured tobacco from said plants has reduced levels of DVTs relative to cured tobacco from plants of the variety designated KY14.
2 more elements
Dependencies are inferred from the claim text.