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US 9,169,511 B2Granted
Isolation of nucleic acids
AssigneeExact Sciences Corporation
InventorsJanelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
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Abstract
Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
Claims16
A kit for isolating a plurality of different target human DNAs from a human stool sample, the kit comprising:
- a) a volume of at least 16 milliliters of a buffer solution for stool homogenization for processing a human stool sample having a mass of at least 4 grams using 4 milliliters of buffer solution per gram of stool;
- b) a spin column comprising a spin filter comprising:
- i) a hollow body (1);
- ii) a bottom end (2);
- and
- iii) an opening (3) at a top end of said hollow body (1), opposite the bottom end (2),
- wherein the hollow body (1) and the bottom end (2) of said spin filter are both composed of the same porous filtering material,
- c) a plurality of different magnetic target-specific capture particles, wherein different target-specific capture particles comprise covalently-attached oligonucleotides complementary to at least a portion of different target human DNAs, wherein said different target human DNAs comprise NDRG4, BMP3, and and KRAS,
- and
- d) guanidine thiocyanate.
7 more elements
Dependencies are inferred from the claim text.