Flux to acetolactate-derived products in lactic acid bacteria
AssigneeButamax Advanced Biofuels LLC
InventorsBrian James Paul, Wonchul Suh
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Abstract
An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed.
Claims11
A recombinant Lactobacillus cell comprising at least one engineered genetic modification that eliminates enzyme activity of endogenously expressed acetolactate decarboxylase, wherein the at least one engineered genetic modification is a deletion of at least a portion of an endogenous gene encoding acetolactate decarboxylase, and at least one engineered genetic modification that eliminates enzyme activity of endogenously expressed lactate dehydrogenase, wherein the at least one engineered genetic modification is a deletion of at least a portion of an endogenous gene encoding lactate dehydrogenase.
Dependencies are inferred from the claim text.