Respiratory syncytial virus (RSV) sequences for protein expression and vaccines
AssigneeUniversity of Massachusetts
InventorTrudy Morrison
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Abstract
The invention provides RSV fusion (F) protein ectodomain polypeptide sequences and nucleotide sequences encoding them, as well as cells containing the invention's polypeptide and nucleotide sequences. The invention further provides VLPs that contain the invention's polypeptides, and methods for using the VLPs for protein expression and vaccine formulation. Also provided are methods for distinguishing between subjects immunized with the invention's compositions and subjects infected with RSV.
Claims19
A recombinant polypeptide sequence comprising a first polypeptide having at least 95% identity to RSV F protein ectodomain polypeptide SEQ ID NO:
- 16, wherein the first polypeptide:
- (a) contains an amino acid other than glutamine at a position corresponding to amino acid 101 of SEQ ID NO:
- 16, contains valine at a position corresponding to amino acid 203 of SEQ ID NO:
- 16, and contains lysine at a position corresponding to amino acid 66 of SEQ ID NO:16, or
- (b) contains an amino acid other than lysine at a position corresponding to amino acid 66 of SEQ ID NO:
- 16, contains valine at a position corresponding to amino acid 203 of SEQ ID NO:
- 16, and contains glutamine at a position corresponding to amino acid 101 of SEQ ID NO:16.
Dependencies are inferred from the claim text.