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US 9,163,278 B2Granted
Isolation of nucleic acids
AssigneeExact Sciences Corporation
InventorsJanelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
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Abstract
Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
Claims16
A system for isolating a target human DNA from a human stool sample, the system comprising:
- a) a volume of stool homogenization solution suitable for processing a human stool sample having a mass of at least 4 grams;
- b) an inhibitor-adsorbing composition comprising insoluble polyvinylpolypyrrolidone particles;
- c) a spin filter, comprising
- i) a hollow body;
- ii) a bottom end;
- and
- iii) an open top end opposite the bottom end,
- wherein the hollow body of said spin filter is made from a porous filtering material;
- d) guanidine thiocyanate;
- and
- e) a plurality of different magnetic target-specific capture particles, wherein different target-specific capture particles comprise covalently-attached oligonucleotides complementary to at least a portion of different target human DNAs, and wherein said different target human DNAs comprise NDRG4, BMP3, and KRAS.
8 more elements
Dependencies are inferred from the claim text.