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US 9,163,262 B2Granted
In vitro and in vivo delivery of genes and proteins using the bacteriophage T4 DNA packaging machine
AssigneeThe Catholic University of America
InventorVenigalla B. Rao
Life of this patent
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Abstract
Described is T4 DNA packaging machine comprising: one or more DNA molecules packaged in a head of the T4 DNA packaging machine, one or more Hoc-fused proteins displayed on the head of the T4 DNA packaging machine, and one or more Soc-fused proteins displayed on the head of the T4 DNA packaging machine. Also described are methods of making and using such a T4 DNA packaging machine.
Claims20
A method comprising the following steps:
- packaging one or more DNA molecules into a Soc−Hoc− phage T4 head to form a DNA packaged head, and
- exposing the DNA packaged head to one or more Hoc fusion proteins and/or one or more Soc fusion proteins to form a phage T4 DNA packaging machine that comprises the DNA packaged head and the one or more Hoc fusion proteins and/or the one or more Soc fusion proteins displayed on surface of the DNA packaged head,
- wherein the Soc−Hoc− phage T4 head retains a portion of a genome DNA and is bound by a gp17 packaging motor, and
- wherein the one or more Hoc fusion proteins comprise a dendritic cell specific ligand CD40L fused to Hoc and the one or more Soc fusion proteins comprise a dendritic cell specific ligand CD40L fused to Soc.
1 more element
A product comprising a phage T4 DNA packaging machine comprising:
- a Soc−Hoc− phage T4 head that retains a portion of a genome DNA and is bound by a gp17 packaging motor,
- one or more DNA molecules packaged in the Soc−Hoc− phage T4 head,
- one or more Hoc fusion proteins and/or one or more Soc fusion proteins displayed on the surface of the Soc−Hoc− phage T4 head,
- wherein the one or more Hoc fusion proteins comprise a dendritic cell CD40 ligand fused to Hoc and the one or more Soc fusion proteins comprise a dendritic cell CD40 ligand fused to Soc.
1 more element
A method comprising:
- packaging one or more DNA molecules into a Soc−Hoc− phage T4 head to form a DNA packaged head, and
- exposing the DNA packaged head to one or more Hoc fusion proteins and/or one or more Soc fusion proteins to form a phage T4 DNA packaging machine that comprises the DNA packaged head and the one or more Hoc fusion proteins and/or the one or more Soc fusion proteins displayed on surface of the DNA packaged head,
- wherein the Soc−Hoc− phage T4 head retains a portion of a genome DNA and is bound by a gp17 packaging motor,
- wherein the one or more Hoc fusion proteins comprise a Hoc-GG fusion protein comprising an IgG binding domain of protein G fused to Hoc,
- wherein the one or more Soc fusion proteins comprise a Soc-GG fusion protein comprising an IgG binding domain of protein G fused to Hoc, and
- wherein a dendritic cell specific receptor monoclonal antibody is displayed on the surface of the DNA packaged head via binding to the Hoc-GG fusion protein and/or the Soc-GG fusion protein.
3 more elements
A method comprising:
- packaging one or more DNA molecules into a Soc−Hoc− phage T4 head to form a DNA packaged head, and
- exposing the DNA packaged head to one or more Hoc fusion proteins and/or one or more Soc fusion proteins to form a phage T4 DNA packaging machine that comprises the DNA packaged head and the one or more Hoc fusion proteins and/or the one or more Soc fusion proteins displayed on surface of the DNA packaged head,
- wherein the Soc−Hoc− phage T4 head retains a portion of a genome DNA and is bound by a gp17 packaging motor, and
- wherein the one or more Hoc fusion proteins comprise a Hoc-CPP fusion protein and the one or more Soc fusion proteins comprise a Soc-CPP fusion protein.
1 more element
Dependencies are inferred from the claim text.