Targeting pseudotyped retroviral vectors
AssigneeThe Regents of the University of California
InventorsIrvin S. Y. Chen, Kouki Morizono
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Abstract
The present invention provides targeted lentiviral vectors that are psuedotyped with mutated Sindbis envelopes. For example, mutations in the Sindbis E2 protein are used to alter viral titer, specificity, specificity index, tropism, and susceptibility to host immune response. Typically, one or more of the E1, E2, or E3 proteins can be mutated at one or more amino acid positions. The psuedotyped, targeted lentiviral vectors of the invention are used to transduce heterologous genes into a cell and can be used for in vivo and ex vivo therapeutic applications, as well as for diagnostic and research tool applications.
Claims13
An isolated pseudotyped retrovirus comprising mutant Sindbis virus E2 and E3 proteins, wherein the sequence SLKQ of the Sindbis virus E2 protein is substituted or deleted and wherein the sequence RSKR of the Sindbis virus E3 protein is substituted or deleted.
An isolated pseudotyped retrovirus comprising mutant Sindbis virus protein E2 and E3, wherein the sequence SLKQ of the Sindbis virus E2 protein is substituted with the amino acid sequence AAAA and wherein the sequence RSKR of the Sindbis virus E3 protein is deleted.
Dependencies are inferred from the claim text.