Gene disruptants producing fatty acyl-CoA derivatives
AssigneeShell Oil Company
InventorsDouglas A. Hattendorf, Jennifer L. Shock, Louis Clark
Life of this patent
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Abstract
This invention provides microbial organisms, particularly yeasts such as Yarrowia lipolytica, that have one or more disrupted genes. The gene disruption(s) may yield improved production of fatty acyl-CoA derivatives.
Claims14
A Y. lipolytica recombinant yeast cell comprising:
- (a) at least one genetic modification in the YALI0C17545 endogenous gene and at least one genetic modification in the YALI0E28534 endogenous gene;
- and (b) an exogenous gene encoding a fatty acyl reductase (FAR) protein, wherein the exogenous gene is operably linked to a promoter, wherein the FAR protein has at least 80% amino sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, 4, 6, and 65-73, wherein the at least one genetic modification comprises:
- (i) a deletion of all or a portion of the coding region of the endogenous gene;
- (ii) a mutation in the endogenous gene such that the gene encodes a polypeptide having reduced activity;
- (iii) a modified regulatory sequence that reduces expression of the endogenous gene;
- or (iv) any combination of (i)-(iii).
An isolated Y. lipolytica recombinant yeast cell comprising (i) an exogenous gene encoding a fatty acyl reductase (FAR) protein, wherein the exogenous gene is operably linked to a promoter, wherein the FAR protein has at least 80% amino sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 2, 4, 6, and 65-73, and (ii) at least one genetic modification in each of the two or more endogenous genes selected from:
- (a) the YALI0C17545 and YALI0E28534 endogenous genes;
- (b) the YALI0C17545, YALI0E28336, and YALI0E28534 endogenous genes;
- (c) the YALI0C17545, YALI0E28534, and YALI0B17512 endogenous genes;
- and
- (d) the YALI0C17545, YALI0E28336, YALI0E11099, and YALI0E28534 endogenous genes,
- wherein the at least one genetic modification comprises:
- (A) a deletion of all or a portion of the coding region of the endogenous gene;
- (B) a mutation in the endogenous gene such that the gene encodes a polypeptide having reduced activity;
- (C) a modified regulatory sequence that reduces expression of the endogenous gene;
- or (D) any combination of (A)-(C).
An isolated Y. lipolytica recombinant yeast cell comprising at least one genetic modification in the YALI0C17545 endogenous gene and at least one genetic modification in the YALI0E28534 endogenous gene;
- wherein the at least one genetic modification comprises:
- (i) a deletion of all or a portion of the coding region of the endogenous gene;
- (ii) a mutation in the endogenous gene such that the gene encodes a polypeptide having reduced activity;
- (iii) a modified regulatory sequence that reduces expression of the endogenous gene;
- or (iv) any combination of (i)-(iii).
Dependencies are inferred from the claim text.