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US 9,157,070 B2Granted
Methods and combination comprising eukaryotic cells and recombinant spider silk protein
AssigneeSpiber Technologies AB
InventorsJan Johansson, Anna Rising, My Hedhammar, Ulrika Johansson, Mona Widhe
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Abstract
A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.
Claims5
A combination, comprising:
- eukaryotic cells;
- and
- a cell scaffold material;
- wherein
- said cell scaffold material comprises a polymer comprising a spider silk protein and a cell-binding motif, said spider silk protein consisting of from 140 to 600 amino acid residues and comprising
- a repetitive fragment of from 70 to 300 amino acid residues derived from the repetitive fragment of a spider silk protein;
- a C-terminal fragment of from 70 to 120 amino acid residues derived from the C-terminal fragment of a spider silk protein;
- and optionally
- an N-terminal fragment of from 100 to 160 amino acid residues derived from the N-terminal fragment of a spider silk protein,
- wherein said cell-binding motif is an oligopeptide coupled to the spider silk protein via at least one peptide bond, and wherein said oligopeptide cell-binding motif is a keratinocyte-specific cell-binding motif.
7 more elements
Dependencies are inferred from the claim text.