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US 9,150,852 B2Granted
Compositions and methods for molecular labeling
AssigneeRaindance Technologies, Inc.
InventorsMichael Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren Roy Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims14
A method of making a barcode library comprising:
- obtaining a first nucleic acid construct wherein the first construct comprises a forward sequence specific primer, a first barcode sequence, and a first universal primer recognition site;
- compartmentalizing the first construct into a fluid compartment;
- introducing a second nucleic acid construct into the fluid compartment, wherein the second construct comprises a reverse sequence specific primer, a second barcode sequence, and a second universal primer recognition site;
- introducing a third construct into the fluid compartment and hybridizing the third construct to the first construct to form a hybrid construct;
- and
- ligating the hybrid construct to form a new construct.
3 more elements
Dependencies are inferred from the claim text.